Quantification of CrAT mRNA levels. (A) TIB-73 murine liver cells were cultivated in DMEM and 10%FCS in comparison to cells treated 24 h with dialyzed FCS. (B) TIB-73 murine liver cells were grown for 24 h with dialyzed FCS and supplemented afterwards for 4 hours with L-carnitine (40–120 μM). (C) Following 24 hours of treatment with dialyzed FCS TIB-73 cells were supplemented with 80 μM L-carnitine for 2–48 h. A second L-carnitine boost (80 μM) took place after 15 hours. Mean value for 0 h L-carnitine suplementation was designated as 1. Supplemented cultures were compared to non-supplemented control (DMEM + 10%diaFCS) in B and C. Values represent means ± SD (n = 3). Means without asterisk show no statistical significance (p > 0.05); (p-values of asterisk marked means are as followed: *p < 0.05, **p < 0.01, ***p < 0.001) (D) TIB-73 cells were grown in DMEM + 10%FCS for 24 hours and afterwards treated with fenofibrate (10–40 μM). Values represent means ± SD (n = 3). Supplemented cultures were compared to physiological control. ***p < 0.001.