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Figure 2 | BMC Genomics

Figure 2

From: Gene Set Enrichment Analysis (GSEA) of Toxoplasma gondii expression datasets links cell cycle progression and the bradyzoite developmental program

Figure 2

Oocyst development induces genes in common with bradyzoite development. A: Gene sets associated with oocyst sporulation. Fritz et al. assayed the transcriptome of oocysts at days 0, 4, and 10 after being expelled by the feline host [10]. We developed five gene sets based upon day(s) of peak expression as described in the materials and methods. The “early” gene set was excluded because too many genes (>500) fell into this group. Additional file 1 shows members of the gene sets and the patterns of expression of oocyst gene sets are shown in Additional file 4. The gradient of color indicates the approximate time of peak expression of the gene sets used (red: early-middle; light green: middle; green: middle-late: blue late; purple: early-late). B: The transcriptome of RH in vitro bradyzoites is enriched for genes associated with middle-stage oocysts. Plotted normalized enrichment scores (NES) for oocyst development gene sets for the datasets reported by Fritz [9]. A positive NES indicates that the gene set is associated with unstressed, tachyzoite parasites, while a negative NES indicates that the gene set is associated with alkaline stressed, in vitro bradyzoites as reported by Lescault [11]. Stars indicate significant enrichment (FWER-adjusted p < 0.05). The color of the bar corresponds to the indicated colors in panel A that depict the approximate time of peak expression of the gene sets used (red: early-middle; light green: middle; green: middle-late: blue late; purple: early-late).

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