Figure 4

Metabolic pathways affected by alkaline stress conditions. A: KEGG pathways affected by bradyzoite induction. Plotted normalized enrichment scores (NES) for tachyzoite and bradyzoite gene sets after GSEA analysis of tachyzoites and in vitro bradyzoites as reported by Lescault [11]. A positive NES indicates that the gene set is associated with unstressed, tachyzoite parasites, while a negative NES indicates that the gene set is associated with stressed, in vitro bradyzoites. Stars indicate significant enrichment (FWER-adjusted p < 0.05). A number of metabolic pathway genes are differentially expressed. B: KEGG pathways affected by deletion of GCN5A. Plotted normalized enrichment scores (NES) for bradyzoite gene sets. A positive NES indicates that the gene set is associated with Δgcn5A mutant RH parasites missing the GCN5A enzyme [12], negative NES indicates that the gene set is associated with the stress parental wild-type RH. Stars indicate significant enrichment (FWER-adjusted p < 0.05).