Figure 1

General genome features of the Aquificales . The genome size is given as the total number of nucleotides in the assembly. Multiple sequence alignments (MSA) were performed by Pomago and TBA. RNAz was applied to the Pomago- and TBA-derived MSAs. De novo protein annotation is based on statistics from BacProt, neglecting previously reported proteins for Aquificales. Annotation of ncRNAs shows the statistics for identified ncRNAs of known function. Details of CRISPR cassettes, number of repeats and associated proteins can be found in Figure 9 and in the Supplemental Material. TmRNAs are classified into two types (Figure 6). The phylogenetic tree shown at the top of the table is based on the whole genome as well as 16S rRNA analysis of the 11 Aquificales species. It reproduces the results presented in [30–32]. For further information, see Supplemental Material. AAE – A. aeolicus, HVI – Hydrogenivirga sp., HTH – H. thermophilus, HBA – Hydrogenobaculum sp., TAL – T. albus, TRU – T. ruber, PMA – P. marina, SAZ – S. azorense, SSP – Sulfurihydrogenibium sp., DTH – D. thermolithotrophum, TAM – T. ammonificans, RS – Riboswitch, WSoP – Weighted sum-of-pairs score [29], * denotes the Hydrogenivirga sp. genome of unfinished assembly.