Analysis of miR156, miR172 and their predicted targets in juvenile and mature cuttings during induction of adventitious root formation. Cuttings were excised, treated with auxin and incubated on rooting tables for the indicated times. RNA samples were prepared and the expression of microRNAs and predicted targets was determined by northern blot and nanostring analysis, respectively from three independent biological repeats. Quantitaive analysis of (a) miR156 expression, and (b) miR172 expression. (c) Representative gels: J, juvenile; M, mature. Quantitative analysis of the predicted targets of (d) miR156 and (e) miR172. Asterisks show statistically significant difference as determined by Scheffe analysis p < 0.05.