Figure 1From: Thermal plasticity of the miRNA transcriptome during Senegalese sole developmentHistogram of read lengths (nt, nucleotides) obtained by SOLiD sequencing of Senegalese sole RNA samples. After trimming, two peaks in read length were found for all samples: one at 22–23 nt and a smaller one at 28–29 nt, which correspond to miRNAs/short interfering miRNAs and piwi-interacting RNAs, respectively. The majority of reads were around the 22 nt peak, indicating miRNA enriched samples. Embryos were incubated at 15°C or 21°C and transferred to 21°C after hatching. The developmental stages analysed were 75% epiboly (75Ep), 20 somites (20S), hatching (hatch) and metamorphosis (met).Back to article page