Table 3 List of primers used in quantification of miRNAs by qPCR
From: Thermal plasticity of the miRNA transcriptome during Senegalese sole development
MicroRNA | Mature sequence (5’ – 3’) | ID qPCR primers (Exiqon) | Amplicon (bp) | Tm (°C) | E (%) |
|---|---|---|---|---|---|
miR-26a | UUCAAGUAAUCCAGGAUAGGCU | 151790-1 | 49 | 69 | 98 |
miR-17a-5p | CAAAGUGCUUACAGUGCAGGUA | 151777-1 | 45 | 71 | 90 |
miR-130c | CAGUGCAAUAUUAAAAGGGCAU | 151782-1 | 48 | 69 | 94 |
miR-206-3p | UGGAAUGUAAGGAAGUGUGUGG | 151786-1 | 45 | 70 | 102 |
miR-199a-5p | CCCAGUGUUCAGACUACCUGUUC | 151813-1 | 46 | 70 | 95 |
miR-181a-5p | AACAUUCAACGCUGUCGGUGAGU | 151802-1 | 46 | 71 | 94 |
miR-181a-3p | ACCAUCGACCGUUGAUUGUACC | 151808-1 | 41 | 71 | 91 |