Figure 4
From: High throughput screening identifies modulators of histone deacetylase inhibitors
Validation of Gcn5 HAT complex components sensitivity to CG-1521. One μL cell suspension was spotted on CG-1521 containing agar plates, containing 25, 55 or 65 μM CG-1521, incubated for 60 h and imaged as described in Methods. The experiment was repeated three times. Gene deletion mutants spt8Δ and spt20Δ are not adjacent, however all deletion mutants are on the same agar plate. Strains lacking Gcn5, Ada2, Hfi1, Ngg1, Sgf29, Sgf73, Spt3, Spt7, Spt8 and Spt20 are sensitive to CG-1521.