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Figure 5 | BMC Genomics

Figure 5

From: Identification of cis-regulatory modules encoding temporal dynamics during development

Figure 5

NR motifs are functional within CRMsNR motifs are functional within CRMs. A) Schematic representation of mutations performed in NR motifs of CG4998 (top) and CG7298 (bottom) CRMs. The location of CRMs (black rectangles) with respect to corresponding genes is indicated on top, and putative NR motifs are represented as grey boxes. NR motifs sequences are indicated below, together with the corresponding mutated sequences. Note that in CG4998-CRM both putative motifs are comprised of two inverted overlapping motifs. Motifs in light grey are of lesser quality and were not analyzed in this study. B) GFP expression pattern at 72 h APF in WT (left) and mutated (right) individuals for CG4998 (top) and CG17298 (bottom) CRMs. A ventral and a dorsal view are shown in each case. NR motif mutations lead to a marked increase of GFP signal. C) Time course of GFP expression between 24 and 96 h APF driven by CG4998 and CG4998-mutated CRMs analyzed by qRT-PCR. GFP expression was normalized to RP49 expression levels and expression ratio relative to GFP expression at 48 h in non-mutated CRM are represented (*p < 0.05; **p < 0.001, Student t-test). C’) The dynamics of GFP expression in the mutated CG4998 CRM is significantly different from the one observed with wild type CRM (*p < 0.05 χ2 test).

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