Figure 6

Eip75B impacts on CG15545-GFP expression. A) Expression dynamics of NR in the cardiac tube during its remodeling from Zeitouni et al. 2007 [16] time course microarray data. Only those whose expression level is above the detection limit at at least one time point were considered. Expression values after within-array and between-array normalization are plotted. B) Expression of these NRs in whole pupae according to flybase modENCODE_mRNA-Seq_U data (http://flybase.org/reports/FBlc0000085.html). Note the particular feature of Eip75B and Hr46 expression which peak at 48 h APF. C) Effect of Eip75B ubiquitous knock down on CG15545-GFP dynamic expression. White prepupae were selected and grown at 25°C (permissive temperature) for 25 hours and shifted at 29°C (restrictive temperature) for 18 h (t0) to 24 h (t + 6 h). Left: expression of GFP in Tub-Gal4, Gal80ts; CG15545-GFP control individuals (WT). Right: expression of GFP in UAS > Eip75BRNAi Tub-Gal4, Gal80ts; CG15545-GFP individuals. Eip75B knock down induces a precocious activation of CG15545-GFP expression noticeable at t0 and lead to increased GFP expression at t3 and t6. Representative individuals. All (30) animals examined displayed this precocious activation of GFP (see Material and Methods).