Figure 5
Temporal expression analysis of the perforin gene in spleen (A), liver (B) and gill (C) tissues of L. rohita hours (h) and days (d) after infection with A. hydrophila . Fold expression was calculated as 2-∆∆Cq. The control group (0 hour post-challenge) was used for calibration. Bars bearing different superscript are significantly different (P < 0.05).