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Table 4 Suggestive and significant QTL for trait hours of survival after challenge with A. hydrophila detected using PLINK (QFAM) and GenAbel (FASTA and GRAMMA) analyses in 21L. rohita families

From: A linkage map of transcribed single nucleotide polymorphisms in rohu (Labeo rohita) and QTL associated with resistance to Aeromonas hydrophila

LG

Pos

SNP

Test

N

Effect

Stat

P-value

Sig

GeneID

1

37.3

55086_181

FASTA

963

-2.22(0.82)

7.32

0.0068

 

Small heat shock

1

37.3

55086_181

GRAMMA

963

-1.18 (0.56)

4.47

0.00538

 

Small heat shock

2

43.9

121615_93

QFAM

1021

 

-1.449

0.00675

  

2

47.4

6937_74

QFAM

1022

 

1.48

0.00772

  

2

48.4

61599_86

QFAM

1022

 

1.48

0.00708

  

2

48.4

83153_20

QFAM

1022

 

1.48

0.00775

  

3

20.3

68284_87

GRAMMA

979

-1.12 (0.57)

3.85

0.00979

  

4

0

84080_123

GRAMMA

978

1.05 (0.5)

4.4

0.00575

  

4

0

84080_123

QFAM

1021

 

1.708

0.00232

  

4

19.7

65405_244

FASTA

979

-2.07(0.73)

7.95

0.00482

  

4

19.7

65405_244

GRAMMA

979

-1.26 (0.55)

5.21

0.00268

  

4

46.8

110358_408

FASTA

979

2.18(0.71)

9.33

0.00226

 

c-type natriuretic peptide 1 precursor

4

46.8

110358_408

GRAMMA

979

1.3 (0.53)

5.96

0.00131

*

c-type natriuretic peptide 1 precursor

4

46.8

110358_408

QFAM

1022

 

2.058

0.00867

 

c-type natriuretic peptide 1 precursor

5

9.6

4797_109

FASTA

978

-1.57(0.55)

8.06

0.00452

  

5

9.6

4797_109

GRAMMA

978

-0.92 (0.41)

5.06

0.00307

  

5

23.8

83820_94

FASTA

979

-2.05(0.72)

8.14

0.00434

 

Brain specific kinase 146

5

23.8

83820_94

GRAMMA

979

-0.97 (0.48)

4.12

0.00753

 

Brain specific kinase 146

5

38.3

1886_343

FASTA

979

1.71(0.64)

7.24

0.00712

 

loc100037090 protein

5

38.3

1886_343

QFAM

1022

 

1.826

0.00577

 

loc100037090 protein

5

43.4

53540_630

FASTA

976

2.76(0.92)

9.03

0.00265

 

protein phosphatase regulatory subunit 10

5

43.4

53540_630

GRAMMA

976

1.28 (0.59)

4.71

0.00429

 

Protein phosphatase Regulatory subunit 10

5

43.4

75322_75

QFAM

1022

 

-1.517

0.00759

  

7

23.4

62374_157

FASTA

968

-3.59(1.04)

11.99

0.00053

*

 

7

23.4

62374_157

GRAMMA

968

-2.26 (0.8)

7.99

0.0002

**

 

7

23.4

62374_157

QFAM

1016

 

-4.763

0.0041

  

7

53.7

87974_385

GRAMMA

979

-0.95 (0.48)

3.96

0.00886

 

sec14-like 1 (cerevisiae)

8

23.3

136056_232

GRAMMA

979

-1.04 (0.47)

4.89

0.00362

  

8

54.9

31265_40

QFAM

1022

 

1.369

0.00338

 

cd22 antigen

10

29.6

82862_249

FASTA

979

-1.99(0.73)

7.32

0.00683

  

10

29.6

82862_249

GRAMMA

979

-1.11 (0.53)

4.39

0.00582

  

12

31.1

57295_319

QFAM

1022

 

-1.117

0.00924

  

12

37.8

52515_4545

QFAM

1022

 

1.667

0.0088

 

titin a

12

37.8

63497_114

QFAM

1022

 

1.667

0.009

 

Unnamed protein product [Tetraodon nigroviridis]

13

19.8

64435_287

QFAM

1022

 

1.813

0.00376

 

Proline rich 5

14

1.9

132996_241

QFAM

1021

 

1.646

0.00159

*

 

14

12.5

112228_90

FASTA

979

2.09(0.65)

10.32

0.00132

*

 

14

12.5

112228_90

GRAMMA

979

1.02 (0.44)

5.43

0.00216

  

14

24.1

96692_167

FASTA

979

1.53(0.59)

6.79

0.00918

 

Novel protein prickle-like family

14

43.9

113696_50

FASTA

976

-3.39(1.15)

8.75

0.0031

 

Perforin 1 (pore forming protein)

14

43.9

113696_50

GRAMMA

976

-2.22 (0.91)

5.92

0.00137

*

Perforin 1 (pore forming protein)

15

1.1

13736_229

GRAMMA

979

-1 (0.5)

4.01

0.00844

  

15

12.5

55466_266

QFAM

1022

 

1.383

0.00078

*

sept2 protein

15

26.7

110434_333

FASTA

979

-1.93(0.75)

6.72

0.00956

 

t cell antigen receptor alpha chain c region

15

26.7

110434_333

GRAMMA

979

-1.18 (0.56)

4.39

0.00584

 

t cell antigen receptor alpha chain c region

15

28.9

54100_91

FASTA

979

2.06(0.68)

9.11

0.00255

 

cerebellin 1 precursor

15

28.9

60130_224

FASTA

978

-1.95(0.66)

8.7

0.00318

  

15

28.9

60130_224

GRAMMA

978

-0.99 (0.46)

4.71

0.00429

  

15

54.4

94824_55

QFAM

1022

 

-1.559

0.00057

*

Adaptor-related protein complex mu 1 subunit

16

23.8

115737_104

QFAM

1020

 

-1.206

0.0094

 

mucin-5b precursor (mucin 5 subtype tracheobronchial) (high molecular weight salivary mucin mg1) (sublingual gland mucin)

16

39.2

136477_76

QFAM

1022

 

2.121

0.00687

 

sp2 transcription factor

18

8.4

60684_109

GRAMMA

978

-0.89 (0.45)

3.88

0.00958

  

18

48.9

52766_1600

FASTA

979

-1.8(0.69)

6.81

0.00909

 

solute carrier family 25 (mitochondrial carrier phosphate carrier) member 25

18

49.9

116665_768

FASTA

979

1.95(0.63)

9.62

0.00193

*

ptc7 protein phosphatase homolog (cerevisiae)

18

49.9

116665_768

GRAMMA

979

1.14 (0.46)

6.04

0.00122

*

ptc7 protein phosphatase homolog (cerevisiae)

18

49.9

13427_160

FASTA

979

2.06(0.67)

9.48

0.00208

  

18

49.9

13427_160

GRAMMA

979

1.13 (0.48)

5.58

0.00188

*

 

19

23.8

111569_63

FASTA

979

2.49(0.77)

10.44

0.00123

*

tbt-binding protein

19

23.8

111569_63

GRAMMA

979

1.35 (0.55)

6.07

0.00119

*

tbt-binding protein

20

0.8

54931_324

GRAMMA

979

1.16 (0.59)

3.86

0.0097

 

solute carrier family member 34

20

3.3

134730_80

FASTA

970

-2.64(0.69)

14.81

0.00012

**

 

20

3.3

134730_80

GRAMMA

970

-0.99 (0.41)

5.89

0.00141

*

 

20

3.3

134730_80

QFAM

1013

 

-2.504

0.0047

  

20

7.9

88771_72

FASTA

954

-1.53(0.58)

6.89

0.00866

  

20

9.4

103839_124

FASTA

970

2.22(0.81)

7.54

0.00603

  

20

9.4

103839_124

GRAMMA

970

1.07 (0.54)

3.86

0.00974

  

20

9.4

110140_1196

FASTA

979

1.6(0.6)

7.06

0.00787

 

glycerol-3-phosphate dehydrogenase

20

9.4

110140_1196

GRAMMA

979

0.82 (0.42)

3.9

0.00938

 

glycerol-3-phosphate dehydrogenase

20

11.1

59816_21

QFAM

1022

 

-1.607

0.00152

*

novel protein vertebrate stabilin 2

20

20.3

134434_222

FASTA

978

-2.03(0.76)

7.09

0.00776

  

20

21.6

20086_69

QFAM

1022

 

1.556

0.00996

 

vacuolar protein sorting 4b

21

47.1

111636_59

QFAM

1022

 

1.417

0.0044

 

kiaa1219 protein

21

51.1

54579_132

FASTA

979

1.63(0.55)

8.68

0.00321

  

21

51.1

54579_132

GRAMMA

979

1.04 (0.43)

5.95

0.00133

*

 

22

6

88524_52

FASTA

979

1.55(0.57)

7.31

0.00687

  

22

6

88524_52

GRAMMA

979

0.97 (0.44)

4.9

0.00357

  

22

6.4

94914_114

FASTA

979

2.07(0.68)

9.2

0.00242

  

22

6.4

94914_114

GRAMMA

979

0.86 (0.42)

4.09

0.00775

  

22

22.3

58881_141

QFAM

1022

 

-1.781

0.00352

 

tuberous sclerosis 1

22

23.2

554_399

FASTA

979

-1.89(0.72)

6.94

0.00841

 

dipeptidyl peptidase 7

22

23.2

554_399

GRAMMA

979

-0.98 (0.5)

3.86

0.00974

 

dipeptidyl peptidase 7

23

0

93296_256

FASTA

978

3.71(0.95)

15.3

0.00009

**

loc795887 protein

23

0

93296_256

GRAMMA

978

2.28 (0.72)

10.11

0.00003

***

loc795887 protein

23

0.9

110253_351

FASTA

979

2.35(0.77)

9.27

0.00232

 

novel protein

23

0.9

110253_351

GRAMMA

979

1.27 (0.55)

5.36

0.00232

 

novel protein

23

27.4

64731_210

QFAM

1022

 

1.41

0.00308

  

24

55.9

67606_298

FASTA

979

-3.63(1.14)

10.21

0.0014

*

serine long chain base subunit 1

24

55.9

67606_298

GRAMMA

979

-2.07 (0.83)

6.25

0.00101

*

serine long chain base subunit 1

25

46.1

54056_576

FASTA

979

1.7(0.65)

6.87

0.00876

 

n-ethylmaleimide sensitive fusion protein attachment protein alpha

  1. LG, linkage group; Pos, location on LG in centimorgans; N, number of progeny and parents analysed; Effect, allele substitution effect of the minor allele with standard error in parenthesis (FASTA and GRAMMAS); Stat, test statistic linear regression coefficient for QFAM, chi-square with one degree of freedom for FASTA and GRAMMA analyses; P, point-wise empirical P-value (QFAM) or permuted P-value with one degree of freedom corrected for inflation factor lambda (FASTA and GRAMMA); Sig, significance after Bonferroni correction (*, P < 0.05; **, P < 0.01; ***, P < 0.001). GeneID, closest SNP homology from BLAST. Tests were considered suggestive when P < 0.01 before Bonferroni correction.