Figure 3
From: Cholesterol depletion induces transcriptional changes during skeletal muscle differentiation
Lim domain only protein 7 (LMO7) localizes at the nuclei region of chick myotubes. Chick myogenic cells were grown for 48 hours and double-stained with an anti-LMO7 antibody (green, A, B, D) and the nuclear dye DAPI (blue, C, D). B is an inset of a region of image A. A merged image is shown in D. Note the strong nuclear envelope staining of the anti-LMO7 antibody in multinucleated myotubes (arrows in A), plus a diffuse cytoplasmic fibrillar staining in both mononucleated and multinucleated cells. Scale bar in C represents 20 μm.