Figure 4

Highly conserved motifs present in C2H2-type zinc finger motifs that regulate C2H2 localization in humans and the fly. (A) Structure of the C2H2-type zinc finger motif and the positions of motifs 82, 93, 121, and 129. (B) Sequence logos of the C2H2 motifs observed in all human proteins and those conserved in humans and the fly. (C) Number of C2H2 motifs observed in the genomes of species ranging from yeasts to humans. The colors in the bar plot indicate the lengths of the C2H2 motifs. (D) C2H2 motif sequence synthesized on the basis of the C2H2 motif in human ZNF24 protein. The ZNF24 proteins are known to be phosphorylated at tyrosine and threonine residues. The asterisk indicates a phosphosite. (E) Localization of m1Venus-2xC2H2 and m1CFP in Cos7 cells. The image showing m1Venus-2xC2H2 relative to m1CFP was produced to demonstrate their localization (upper images). Localizations of m1Venus-2xC2H2 and the m1CFP-2xC2H2YF mutant in Cos7 cells. The image of m1Venus-2xC2H2 relative to m1CFP-2xC2H2YF was produced to demonstrate their localization (lower images). The 2xC2H2YF mutant with a YF mutation at the tyrosine residue, which is indicated by an asterisk in Figure 4E. The arrows indicate nuclear locations with higher relative differences in CFP and YFP images.