Table 3 Effect upon fermentation of yeast lacking genes encoding vacuolar H + ATPase complex and associated proteins
Gene | V-ATPase subunit | Function (eight subunit peripheral domain) | Essential for fermentation |
|---|---|---|---|
V1domain | |||
VMA1 (TFP1) | A | ATP hydrolysis | Yes |
VMA2 | B | Regulatory, ATP binding?, Actin binding? | Yes |
VMA5 | C | Stator | Yes |
VMA8 | D | Rotor | Yes |
VMA4 | E | Stator | Yes |
VMA7 | F | Rotor | Yes |
VMA10 | G | Stator | Yes |
VMA13 | H | Stator | Yes |
V o domain | (six subunit integral domain) | ||
VPH1 | a (vph1p) | Proton pore, stator, sorting (vacuole) | Yes |
STV1 | a (Stv1p) | Proton pore, stator, sorting (golgi/endosome) | No |
VMA3 (CUP5) | c | Proton pore, rotor (dicyclohexylcarbodiimide binding) | Yes |
VMA11 (TFP3) | c’ | Proton pore, rotor | Yes |
VMA16 (PPA1) | c” | Proton pore, rotor | Yes |
VMA6 | d | Rotor | Yes |
VMA9 | e | ? | nd |
Assembly Factors | |||
VPH2 (VMA12) | Vph2p | Integral membrane protein; vacuolar H+ ATPase (V-ATPase) assembly | Yes |
VMA22 | Vma22p | Peripheral membrane protein; vacuolar H+ ATPase assembly | Yes |
VPS3 | Vps3p | CORVET tethering complex; cytoplasmic protein required for sorting & processing of soluble vacuolar proteins, acidification of vacuolar lumen, & assembly of V-ATPase | No |
PKR1 | Pkr1p | V-ATPase assembly factor, functions with other V-ATPase assembly factors in ER to assemble V-ATPase membrane sector (Vo) | Yes |
RAVE complex | |||
SKP1 | Skp1p | Evolutionarily conserved kinetochore protein; part of SCF ubiquitin ligase complex, CBF3 complex binding centromeric DNA, & RAVE complex regulating assembly of V-ATPase | nd |
RAV1 | Rav1p | Subunit of RAVE complex (Rav1p, Rav2p, Skp1p), promotes assembly of V-ATPase | Yes |
RAV2 | Rav2p | Subunit of RAVE complex, promotes assembly of V-ATPase | Yes |