Genome-wide identification of the Fermentome; genes required for successful and timely completion of wine-like fermentation by Saccharomyces cerevisiae

Michelle E Walker; Trung D Nguyen; Tommaso Liccioli; Frank Schmid; Nicholas Kalatzis; Joanna F Sundstrom; Jennifer M Gardner; Vladimir Jiranek

doi:10.1186/1471-2164-15-552

Table 3 Effect upon fermentation of yeast lacking genes encoding vacuolar H ⁺ ATPase complex and associated proteins

From: Genome-wide identification of the Fermentome; genes required for successful and timely completion of wine-like fermentation by Saccharomyces cerevisiae

Gene	V-ATPase subunit	Function (eight subunit peripheral domain)	Essential for fermentation
	V₁domain	Function (eight subunit peripheral domain)
VMA1 (TFP1)	A	ATP hydrolysis	Yes
VMA2	B	Regulatory, ATP binding?, Actin binding?	Yes
VMA5	C	Stator	Yes
VMA8	D	Rotor	Yes
VMA4	E	Stator	Yes
VMA7	F	Rotor	Yes
VMA10	G	Stator	Yes
VMA13	H	Stator	Yes
	V _o domain	(six subunit integral domain)
VPH1	a (vph1p)	Proton pore, stator, sorting (vacuole)	Yes
STV1	a (Stv1p)	Proton pore, stator, sorting (golgi/endosome)	No
VMA3 (CUP5)	c	Proton pore, rotor (dicyclohexylcarbodiimide binding)	Yes
VMA11 (TFP3)	c’	Proton pore, rotor	Yes
VMA16 (PPA1)	c”	Proton pore, rotor	Yes
VMA6	d	Rotor	Yes
VMA9	e	?	nd
	Assembly Factors
VPH2 (VMA12)	Vph2p	Integral membrane protein; vacuolar H⁺ ATPase (V-ATPase) assembly	Yes
VMA22	Vma22p	Peripheral membrane protein; vacuolar H⁺ ATPase assembly	Yes
VPS3	Vps3p	CORVET tethering complex; cytoplasmic protein required for sorting & processing of soluble vacuolar proteins, acidification of vacuolar lumen, & assembly of V-ATPase	No
PKR1	Pkr1p	V-ATPase assembly factor, functions with other V-ATPase assembly factors in ER to assemble V-ATPase membrane sector (V_o)	Yes
	RAVE complex
SKP1	Skp1p	Evolutionarily conserved kinetochore protein; part of SCF ubiquitin ligase complex, CBF3 complex binding centromeric DNA, & RAVE complex regulating assembly of V-ATPase	nd
RAV1	Rav1p	Subunit of RAVE complex (Rav1p, Rav2p, Skp1p), promotes assembly of V-ATPase	Yes
RAV2	Rav2p	Subunit of RAVE complex, promotes assembly of V-ATPase	Yes

Fermentations (100 mL) were conducted in CDGJM_200 as described in Materials and Methods. Deletants which resulted in protracted fermentation were considered essential for fermentation in high sugar media, whilst those which were not affected, were considered non-essential.
VMA9 (YCL005W-A) and SKP1 (YDR328C) are not in the homozygous diploid deletion library. SKP1 is an essential gene for growth.
nd: not determined.

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