VMA1 (TFP1)
|
A
|
ATP hydrolysis
|
Yes
|
VMA2
|
B
|
Regulatory, ATP binding?, Actin binding?
|
Yes
|
VMA5
|
C
|
Stator
|
Yes
|
VMA8
|
D
|
Rotor
|
Yes
|
VMA4
|
E
|
Stator
|
Yes
|
VMA7
|
F
|
Rotor
|
Yes
|
VMA10
|
G
|
Stator
|
Yes
|
VMA13
|
H
|
Stator
|
Yes
|
|
V
o
domain
|
(six subunit integral domain)
| |
VPH1
|
a (vph1p)
|
Proton pore, stator, sorting (vacuole)
|
Yes
|
STV1
|
a (Stv1p)
|
Proton pore, stator, sorting (golgi/endosome)
|
No
|
VMA3 (CUP5)
|
c
|
Proton pore, rotor (dicyclohexylcarbodiimide binding)
|
Yes
|
VMA11 (TFP3)
|
c’
|
Proton pore, rotor
|
Yes
|
VMA16 (PPA1)
|
c”
|
Proton pore, rotor
|
Yes
|
VMA6
|
d
|
Rotor
|
Yes
|
VMA9
|
e
|
?
|
nd
|
|
Assembly Factors
| |
VPH2 (VMA12)
|
Vph2p
|
Integral membrane protein; vacuolar H+ ATPase (V-ATPase) assembly
|
Yes
|
VMA22
|
Vma22p
|
Peripheral membrane protein; vacuolar H+ ATPase assembly
|
Yes
|
VPS3
|
Vps3p
|
CORVET tethering complex; cytoplasmic protein required for sorting & processing of soluble vacuolar proteins, acidification of vacuolar lumen, & assembly of V-ATPase
|
No
|
PKR1
|
Pkr1p
|
V-ATPase assembly factor, functions with other V-ATPase assembly factors in ER to assemble V-ATPase membrane sector (Vo)
|
Yes
|
|
RAVE complex
| |
SKP1
|
Skp1p
|
Evolutionarily conserved kinetochore protein; part of SCF ubiquitin ligase complex, CBF3 complex binding centromeric DNA, & RAVE complex regulating assembly of V-ATPase
|
nd
|
RAV1
|
Rav1p
|
Subunit of RAVE complex (Rav1p, Rav2p, Skp1p), promotes assembly of V-ATPase
|
Yes
|
RAV2
|
Rav2p
|
Subunit of RAVE complex, promotes assembly of V-ATPase
|
Yes
|