Table 5 Comparison of RNA-seq data with expression data from qRT-PCR
From: Transcriptome analysis of callus from Picea balfouriana
NCBI gene ID | UniProt_ID | Description | Method | Expression level in ET | Expression level in NET | Fold change |
|---|---|---|---|---|---|---|
840475 | Swiss-Prot:Q9LP24 | Leucine-rich repeat receptor-like protein kinase | RNA-seq | Log-cpm in ET | Log-cpm in NET | log2 ratio (ET/NET) |
605.5 | 3640.6 | 2.6 | ||||
qRT-PCR | ET(ΔCT) | NET(ΔCT) | 2-ΔΔCT | |||
9.6 | 4.6 | 2.1 | ||||
817958 | Swiss-Prot:Q6X7J4 | WUSCHEL-related homeobox 9 | RNA-seq | Log-cpm in ET | Log-cpm in NET | log2 ratio (ET/NET) |
155.7412756 | 0.353625 | 8.8 | ||||
qRT-PCR | ET(ΔCT) | NET(ΔCT) | 2-ΔΔCT | |||
3.0 | 1.3 | 3.4 | ||||
831649 | Swiss-Prot:Q8GY25 | WUSCHEL-related homeobox 12 | RNA-seq | Log-cpm in ET | Log-cpm in NET | log2 ratio (ET/NET) |
136.2 | 1.8 | 6.3 | ||||
qRT-PCR | ET(ΔCT) | NET(ΔCT) | 2-ΔΔCT | |||
0.7 | 0.1 | 1.5 |