Figure 3

miRNA sequence variation. (A-B) 5’- and 3’-end sequence heterogeneity of the 5p- and 3p-derived reads as indicated. (C) Distribution of the predominant sequence reads grouped by their 5’-ends compared with the miRNAs reported in miRBase v19. (D) Frequency of sequence reads grouped by their 3’-end compared to the predominant miRNA read. (E) Sequence compilations of five highly abundant mosquito miRNAs showing their mature sequence including the corresponding 3’-end sequence variations. Below, the mature miRNA sequence reported in miRBase is shown in green, adjacent genomic sequence is in black. (F-G) 3‘-end extensions of the 5p- and 3p-sequences in sugar and blood-fed mosquitoes as indicated. (H) The percentage of A- and U-tailed sequence reads of the extremely abundant miRNAs.