Figure 7
Membrane transport of R6G is reduced in each TF mutant and relative mtDNA copy number is less in dpb4 ∆ mutant (7B). (A) Parental (SN250) and each mutant were assayed for transport of R6G. Relative RFU, relative fluorescent units over a 90 min time interval were determined. Cells were starved in buffer for 20 min then glucose was added to each culture. Compared to parental cells, all mutants had little transporter activity. (B) The ratio of mtDNA copy number to that of nuclear DNA (nDNA) is calculated by ΔCt with matched pairs of mtDNA/nDNA primers. The relative copy number of mtDNA is averaged from three biological replicates (mtDNA/nDNA). Compared to the parental strain, dpb4 Δ has less mtDNA copies compared to its own nDNA or parental nDNA. However mutants (rbf1Δ and hfl1Δ) have a similar mtDNA copy number as the parental strain. (mumtDNA: mutant mtDNA; wtnDNA: wild type strain nDNA).