Extensive structural variations between mitochondrial genomes of CMS and normal peppers (Capsicum annuum L.) revealed by complete nucleotide sequencing

Table 1 General features of mitochondrial genomes of two pepper lines and one tobacco line

Features	FS4401	Jeju	Nicotiana tabacum
Genome size (bp)	507,452	511,530	430,597
GC content (%)	44.5	44.6	45.0
Coding sequences (bp)^a	40,085 (7.9%)	39,524 (7.7%)	43,642 (10.1%)
Plastid-derived sequences (bp)^b	59,873 (11.8%)	64,815 (12.7%)	19,492 (4.5%)
Repeated sequences (bp)^c	42,505 (8.4%)	70,122 (13.7%)	73,511 (17.1%)
Gene content (number)	66	64	61
Protein coding genes^d	38	37	37
rRNAs	3	3	3
tRNAs^e	25 (13)	24 (12)	21 (9)

^aAll of the copies of duplicated genes were included.
^bComplete sequences of chloroplast genomes of FS4401 [33] and Nicotiana tabacum [34] were used to screen for plastid-derived sequences in mitochondrial genomes of FS4401/Jeju and N. tabacum, respectively, based on the BLASTN algorithm. The value for N. tabacum was different from that of Sugiyama et al. [35] probably due to differences in methodology used to isolate plastid-derived sequence.
^cSequences longer than 100 bp and showing similarity higher than 95% between copies were considered repeated. In case repeated sequence units were overlapping, nucleotides that were included in repeated sequences at least one time were counted without repetition.
^drpl10, which was reported to be a conserved mitochondrial gene by Kubo and Arimura [32] was added to the list of genes described by Sugiyama et al. in tobacco [35].
^eThe number of tRNA genes included in plastid-derived sequences is given in parenthesis.
The tobacco line is ‘Bright Yellow 4′ for which the mitochondrial genome was analyzed by Sugiyama et al. [35].

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