Figure 8

Western blot analysis of 14-3-3 zeta and PDI. Hemolymph proteins samples of honey bee worker (A. m. ligustica and A. c. cerana) larvae and pupae on days 1, 3 and 5 were subjected to SDS-PAGE followed by Western blot analysis. 14-3-3 zeta and PDI were detected using corresponding antibodies. Beta-tubulin was used as reference control. (A) The Western blot images of 14-3-3 zeta, PDI and beta-tubulin. (B) The relative fold change of 14-3-3 zeta and PDI (normalized by beta-tubulin). The gray bars represent relative fold change of protein expression. Error bar is standard deviation (p < 0.05).