Figure 1

Schematic of RNA-seq library generation protocols. RNA fragments are ligated to adaptors that permit reverse transcription and PCR amplification prior to sequencing. (A) The CircLig protocol involves ligation to a ssDNA adaptor prior to reverse transcription with a primer that results in PCR primer sites either side of the fragment when the cDNA is circularised. (B) The Life Technologies protocol involves hybridisation to duplexed RNA-DNA adaptors containing degenerate ssDNA overhangs prior to ligation.