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Figure 4 | BMC Genomics

Figure 4

From: Evaluating bias-reducing protocols for RNA sequencing library preparation

Figure 4

Position specific bias associated with Mth K97A. The abundance of each nucleotide at each position within the degenerate portion of the sequenced reads was calculated. (A) The use of Mth K97A in library construction was associated with pronounced over-representation of A and C at the 3rd nucleotide from the ligation site. (B) Sequences were ranked based on abundance and the nucleotide content calculated across a sliding window of 1000 sequences.

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