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Table 4 Genes used for qPCR in the liver and white muscle to confirm the results of the microarray

From: Differential gene expression in small and large rainbow trout derived from two seasonal spawning groups

Liver

Gene name

Forward primer

Reverse primer

IGF-binding protein 1 precursor (IGF1BP)

5′-CCAAGCAGTGTGAGTCGTCTCT-3′

5′-CCGGAATCTTCTTCCCATT -3′

Complement C1q-like protein 4 precursor (C1QL4 pre)

5′-GCAGGCACTGAAAGACATTCC-3′

5′-TGCCTTTTGGAGTCCATTGC-3′

Hemoglobin subunit alpha-1 (HBA1)

5′-TGGATTGTAAACACATCGTTCGT-3′

5′-CCACTATCAGTAGCACTGTCAAAGC-3′

Muscle

Max protein (MAX)

5′-TGTCGATGGTCTGGGACAACT-3′

5′-TCCCCGCCCTGGATTT-3′

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

5′-TATGACTCCACCCACGGTGTT-3′

5′-TGCCAATGATCAGCTTTCCA-3′

ADP/ATP translocase 2 (ANT2)

5′-TTTCCATTGAGCCCCTTTCA-3′

5′-ACGACCGTGGAAATGTTTGTAA-3′

Control

  

Beta-actin*

5′-CAGCCCTCCTTCCTCGGTAR-3′

5′-AGCACCGTGTTGGCGTACA-3′

  1. *Beta-actin serves as the control gene for both the liver and muscle genes.