Data validation using HPLC and Real Time PCR analysis. (A) Estimation of triterpenoid content in the leaves of O. sanctum and O. basilicum. (B) Validation of the expression pattern of selected pathway genes was carried out using total RNA isolated from O. sanctum and O. basilicum leaf tissues through quantitative Real time PCR. Error bars represent standard deviation between three replicates. (C) Digital gene expression of PAL, CCR, CS3′H, EGS, CVOMT, HPPR, BAS, PMK.