Figure 3

Microscopic analysis of Aspergillus nidulans mycelia in controls (top panels) or on suberin (bottom panels) at the first time point, showing the red safranin stain (scale bar: 137 μm) (a), the hyphal morphology (detected by SEM, scale bar: 10 μm) (b), and the total (c) and the dead (d) hyphae, shown in blue (calcofluor white stain) and red (propidium iodide stain) (scale bar: 1000 μm). Only the controls showed the typical features of fungal biofilms, namely the extracellular matrix stained with safranin and the hyphal alignment. Total and dead hyphae were alike in controls and on suberin.