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Table 2 List of the extracellular protein species identified in the secretome of Aspergillus nidulans at the last time point of growth on suberin

From: Elucidating how the saprophytic fungus Aspergillus nidulans uses the plant polyester suberin as carbon source

Gene number

Description

MW [kDa]

calc. pI

SignalPa

SwissProt

NCBI

Microarray data*

Total peptides

Cov (%)

Total peptides

Cov (%)

Firstc

Midc

Lastc

Plant Polymer Degrading Enzymes

AN5309

Cutinase (Cut1)

22.4

7.3

Y

3

12.21

2

9.09

305.8

-3

1.5

AN8046

Triacylglycerol lipase

31.3

4.6

Y

-b

-b

7

47.28

10.4

-1.6

2.7

AN3613

β-1,4-xylanase (XlnA)

24.1

6.3

Y

4

36

4

36

6.5

-2.2

-1.1

AN7401

β-1,4-endoxylanase (XlnE)

37.7

5.5

Y

-b

-b

3

11.83

-1.3

-1.4

1

AN1818

β-1,4-xylanase C (XlnC)

35.4

5.2

Y

5

20.49

-b

-b

1.2

-1.2

-1.3

AN8477

β -1,4-xylosidase

60.3

5.4

N

-b

-b

4

9.71

1.6

1.1

1.1

AN2828

β-glucosidase L (BglL)

77.8

4.8

Y

14

21.71

12

22.52

-1.2

-1.3

2.4

Fungal Development

AN0472

β-1,3-endoglucanase A (EngA)

97.8

5.7

Y

-b

-b

6

8.49

5.8

1.1

1.2

AN7950

β-1,3-endoglucosidase (EglC)

46.7

4.6

Y

10

28.39

10

28.39

-d

-d

-d

AN4825

β-1,3-glucosidase

96.5

5.5

Y

-b

-b

11

17

42.5

1.1

1.2

AN2395

β-glucuronidase

68.5

4.8

Y

-b

-b

14

28.06

7.4

-1.4

1.2

AN4871

Chitinase B (ChiB)

44.2

5.6

Y

-b

-b

34

80.65

12.7

1.4

1.1

AN2017

α-glucosidase (AgdA)

109.6

5.2

Y

-b

-b

6

8.17

-1.5

-1.9

2.3

AN8445

Aminopeptidase Y

53.7

6.8

Y

-b

-b

6

19.68

1.8

-1.5

1.6

AN2366

Serine protease

25.4

4.4

Y

-b

-b

3

17.27

-d

-d

-d

AN5558

Alkaline serine protease (PrtA)

42.2

5.3

Y

8

32.75

7

32.75

5.4

1.3

1.1

AN7962

Metalloproteinase (PepJ)

37.4

5.1

N

6

18.36

6

18.36

278.8

1.8

1.2

AN4245

Ceramidase

80

5

Y

-b

-b

7

14.8

1.6

1.5

1.8

AN9339

Catalase B (CatB)

79.1

5.1

Y

23

35.6

20

34.54

-2.4

1.8

1

Miscellaneous

AN3351

Uncharacterised

63.2

5.1

Y

-b

-b

5

12.91

1.6

-1.4

-1.1

AN3246

Uncharacterised

22.3

6.4

N

-b

-b

4

29.8

2

-1.3

2

AN6273

Allergenic Asp F13

16.3

4.8

Y

-b

-b

3

32.91

-1

-1.5

-1.4

AN5879

Phosphatidylglycerol/phosphatidylinositol transfer protein

18.3

5

Y

9

53.25

9

53.25

1

1

-1

AN8979

Alcohol dehydrogenase I (AlcA)

36.9

6

N

5

25.57

5

25.57

-15.1

-8.8

1

AN8043

Uncharacterised

16.9

4.8

N

-b

-b

3

26.42

-4

-2

-1

  1. aSignalP was used to predict secretion signals [67, 68] and bnot found in the database search. Corresponding microarray data are shown for comparison. *Values highlighted in bold have |FC| > 2 and p-value < 0.01 in the microarray data; cFold changes (FCs) in pair-wise comparison of consecutive time points; dnot represented in the chip.