Elucidating how the saprophytic fungus Aspergillus nidulans uses the plant polyester suberin as carbon source

BMC Genomics

Table 3 q RT-PCR analysis of a selected set of genes coding putative lipid hydrolysing enzymes or major regulatory proteins

Carbon source	Gene	Encoded protein	qRT-PCR			Microarray*
			First^d	Mid^d	Last^d	First^d	Mid^d	Last^d
Suberin	AN6195	CreA	8.3	-2.3	-1.9	4.3	-1.4	-1.6
	AN1052	VeA	2.5	-1.0	-1.0	2.6	-1.3	1.0
	AN7050	FarA	2.3	-1.7	1.3	2.8	-1.7	1.3
	AN5309	Cut1	119.9	-3.1	1.8	305.8	-3.0	1.5
	AN7541	Cut2	-1.5	-1.0	1.5	-1.3	-1.1	1.1
	AN7180	Cutinase	1.2	1.1	1.9	1.3	-1.2	-1.1
	AN5267	FaeC	3.2	-2.1	1.7	3.0	-2.4	1.1
	AN2697	Putative lipase^a	-1.8	1.4	1.8	1.6	-1.7	-1.1
	AN5777	Putative lipase	2.9	1.0	1.7	2.9	-1.2	1.6
	AN8046	Putative lipase	4.1	-1.1	2.3	10.4	-1.6	2.7
	AN8900	Putative lipase^b	-1.8	1.7	1.5	1.6	1.6	-1.1
	AN4748	Uncharacterised protein^c	31.8	-1.8	-3.8	24.6	-1.7	-4.5
Glyceryl tripalmitate	AN5309	Cut1	1.4	36.4	-7.8
	AN7541	Cut2	2.7	-3.8	-1.3
	AN7050	FarA	3.7	-1.7	-1.7
	AN8046	Putative lipase	8.2	2.3	-19.4
Octyl octanoate	AN5309	Cut1	3.3	14.0	-1.6
	AN7541	Cut2	4.1	-1.2	-3.0
	AN7050	FarA	2.7	-1.3	-1.4
	AN8046	Putative lipase	2.8	5.8	-11.7

Values represent the relative expression of selected genes in pair-wise comparisons of consecutive time points. The expression of each gene was normalised by the expression of the 60S ribosomal protein L33-A gene (AN2980).
^*Values highlighted in bold have |FC| > 2 and p-value < 0.01 in the microarray data; ^acontains feruloyl esterase and tannase domains, high homology with feruloyl esterase B in N. crassa; ^borthologue of A. niger An09g02270, which encodes a triacylglycerol lipase; ^corthologue of S. cerevisiae NOP6, which is necessary for rRNA-binding protein required for 40S ribosomal subunit biogenesis.^dFold changes (FCs) in pair-wise comparison of consecutive time points. Corresponding microarray data are shown for comparison.

ISSN: 1471-2164