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Table 5 Gene-specific primers and amplicon sizes in qRT-PCR detection of 19 MdMLO -like genes based on Malus domestica cv. Golden Delicious genome sequence

From: Characterization of the MLO gene family in Rosaceae and gene expression analysis in Malus domestica

Gene

Forward primer (5 ′   – 3 ′ )

Reverse primer (5 ′   – 3 ′ )

Size (bp)

Efficiency

MdMLO1

GTGGGCTCGGTCGGCCAAAA

CCAGCACCAGCACCAGAACCA

81

2.06

MdMLO2

CGTTGGATCAACCACTGCGCCT

TGAGCTGCAGCCAGTGGGATCT

87

1.83

MdMLO3

CCACTGCGCCTCTCTGAAGCA

CCACCAAAACGGCTCTCCAGGT

93

2.12

MdMLO4

TGTTGCAGACACTATGCTGCCATGT

GGCAGCAGCTAAAGATCTGCGT

109

1.87

MdMLO5

TCGTCAGGCTCTCATTCGGGGT

GTGCTGCTGCCACTCCCTC

132

1.80

MdMLO6

TTCGCGGAGGAGGGGTCGTT

TTCGAGCGACAGCAACGGCA

72

2.15

MdMLO7

TGGAGCAAGTCACCAGTCTCCAT

CGCTTCCTGGTGCCAAATGTGC

127

2.12

MdMLO8

GTCAAGCTAATCTTACCACGCGCT

GGCTGGAAGGAAGGACAGCCA

85

1.95

MdMLO9

GCTGCAACACGTAATCACCC

AGAACGCCATTTCGAAAGCA

173

2.30

MdMLO10

GCGATCGTTGGCCTTGACTCC

TTCCGCGCTCGACAAGCAGA

86

1.92

MdMLO11

CCGTTCCATCACCAAGACGA

ATTGCTCTCCGAGTTACGCC

102

1.90

MdMLO13

ACATTGTCCCCAGGCTTGTT

GCCCAACCAATAAGTCCCGA

151

2.00

MdMLO14

TGCACTTGTCAGCCAGATGGG

GCATCTCCCACCCACGAACCG

81

2.15

MdMLO15

GCGCCTTTCTCTCTGCTGGGT

CGCGTGCGAGGTGGTCTCTT

90

2.01

MdMLO17

TTGCCACTGTATGCCTTGGT

TGCTTGCTTCTGTGCGAATG

163

2.15

MdMLO18

AAGGAAGGCTCTCATTCAGGCTCT

TGCAATTGGCTTTTGACCAACGGT

100

2.22

MdMLO19

CAGAGTGGCGACTGCACTTA

GGGACATGGAGTGCAAAGGA

110

1.97

MdMLO20

AAAAAGCTCCACCAACCCCA

TTTCTCTCCCATGACGCTCG

165

2.11

MdMLO21

CCTTGTTCGAGGCCGTAGAG

ACCAAGTGCTTTGGTGGTTT

176

1.95

β-actin

CTATGTTCCCTGGTATTGCAGACC

GCCACAACCTTGATTTTCATGC

82

1.90

Ubiquitin

CATCCCCCCAGACCAGCAGA

ACCACGGAGACGAAGCACCAA

349

1.91

Elongation Factor 1

TACTGGAACATCACAGGCTGAC

TGGACCTCTCAATCATGTTGTC

308

2.07