Chst5 knockdown induced a late in myotube formation. Myogenic differentiation of MSC: untreated (Without), treated with plasmid containing shRNA without target in murine genome (shRNA NEG), treated with plasmid containing shRNA for Chst5 (shRNA Chst5). Photos were taken at different time points after hematoxylin/eosin staining. Magnification was 400x (for lower magnification see Additional file 3). Red arrows point nucleus into myotubes.