Chst5 knockdown delayed the cell fusion. Fusion index of MSC cultures seeded at 5×103 cells/cm2 on Matrigel®. Cultures were compared every 24 h after myogenic differentiation induction by serum starvation. Cultures were untreated (black), treated with plasmid contains negative control shRNA (dark grey), treated with plasmid containing shRNA for Chst5 (white), see Methods. Standard errors were calculated using triplicate and the Student t-test determined significant differences between control and treated cultures. **p < 1%; *p < 5%.