Itga11 knockdown reduced myotube formation. Myogenic differentiation of MSC: untreated (Without), treated with plasmid containing shRNA without target in murine genome (shRNA NEG), treated with plasmid containing shRNA for Itga4 (shRNA Itga4) or plasmid containing shRNA for Itga11 (shRNA Itga11). Photos were taken at different time points after hematoxylin/eosin staining. Magnification was 400× (for lower magnification see Additional file 3). Red arrows point myotubes.