Figure 8

Anti-ITGA4 and/or anti-ITGA11 antibodies inhibited cell fusion of myoblasts. A. Experiments were performed on cultures plated on Matrigel® and under differentiation conditions. The five cultures were, untreated (triangles), treated with 1 μg/ml isotypic antibodies as negative control (cross over squares), treated with 1 μg/ml anti-ITGA4 antibodies as positive control (squares), treated with 1 μg/ml anti-ITGA11 antibodies (circles) or treated with 0.5 μg/ml anti-ITGA4 and 0.5 μg/ml anti-ITGA11 antibodies (diamonds). Significance was calculated using the t-test on three simultaneous replicates (see standard bars). ***indicates time with significant difference between WT and all neutralization tests, p <0.1%. B. The specificity of anti-ITGA4 and anti-ITGA11 was controlled by western blot using 50 μg of cellular lysate. MW indicates the molecular weight ladder.