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Table 1 qPCR validation of selected transcripts exhibiting differential accumulation in microarray

From: Differential gene expression and metabolomic analyses of Brachypodium distachyon infected by deoxynivalenol producing and non-producing strains of Fusarium graminearum

   Microarray   qPCR   
Gene Comparison Fold change (Log2) P-val (Bonferroni) Fold change (Log2) Standard error Gene description
Bradi1g75310 Fg don+ vs Tween 6.93 0.00 14.40 0.26 Cytochrome P450 monooxygenase
  Fg don- vs Tween 0.77 1.00 6.28 0.40  
  Fg don+ vs Fg don- 6.16 0.00 8.12 0.26  
Bradi3g22880 Fg don+ vs Tween 7.44 0.00 10.24 0.08 MATE efflux
  Fg don- vs Tween 1.36 0.18 2.26 0.11  
  Fg don+ vs Fg don- 6.08 0.00 7.97 0.08  
Bradi2g41010 Fg don+ vs Tween 9.30 0.00 12.93 0.15 Unknown
  Fg don- vs Tween 4.80 0.00 3.84 0.25  
  Fg don+ vs Fg don- 4.51 0.00 9.08 0.15  
Bradi2g35930 Fg don+ vs Tween 8.19 0.00 10.09 0.07 Glutathione S-transferase GSTF1-like
  Fg don- vs Tween 4.15 0.00 3.46 0.45  
  Fg don+ vs Fg don- 4.05 0.00 6.63 0.07  
Bradi4g39950 Fg don+ vs Tween 2.38 0.00 3.12 0.19 NADP-dependent alkenal double bond reductase P1
  Fg don- vs Tween 5.68 0.00 6.05 0.28  
  Fg don+ vs Fg don- -3.29 0.00 -2.93 0.19  
Bradi4g05040 Fg don+ vs Tween 2.25 0.00 3.35 0.06 PR10
  Fg don- vs Tween 7.48 0.00 8.42 0.27  
  Fg don+ vs Fg don- -5.22 0.00 -5.08 0.06  
Bradi4g36870 Fg don+ vs Tween 3.69 0.00 4.75 0.05 Ornithine decarboxylase
  Fg don- vs Tween 0.90 1.00 1.59 0.50  
  Fg don+ vs Fg don- 2.77 0.00 3.16 0.05  
Bradi4g36810 Fg don+ vs Tween 5.06 0.00 10.14 0.28 Ornithine decarboxylase
  Fg don- vs Tween 1.37 0.15 5.55 0.26  
  Fg don+ vs Fg don- 3.69 0.00 4.59 0.28  
Bradi1g50067 Fg don+ vs Tween 3.63 0.00 5.23 0.16 Arginine decarboxylase
  Fg don- vs Tween 1.16 1.00 1.58 0.31  
  Fg don+ vs Fg don- 2.46 0.00 3.65 0.16  
Bradi3g47110 Fg don+ vs Tween 5.86 0.00 11.99 0.34 Phenylalanine ammonia-lyase
  Fg don- vs Tween 0.32 1.00 5.59 0.59  
  Fg don+ vs Fg don- 5.53 0.00 6.41 0.34  
Bradi3g53227 Fg don+ vs Tween 3.56 0.00 6.94 0.14 3-hydroxy-3-methyl-glutaryl-CoA reductase
  Fg don- vs Tween 0.17 1.00 1.77 0.15  
  Fg don+ vs Fg don- 3.40 0.00 5.17 0.14  
Bradi2g13360 Fg don+ vs Tween 4.10 0.00 5.69 0.14 Mevalonate kinase
  Fg don- vs Tween 1.93 0.00 2.26 0.36  
  Fg don+ vs Fg don- 2.17 0.00 3.43 0.14  
Bradi5g02780 Fg don+ vs Tween 2.41 0.00 4.87 0.16 UDP-glycosyltransferase
  Fg don- vs Tween 1.16 1.00 2.66 0.36  
  Fg don+ vs Fg don- 1.25 1.00 2.21 0.16  
Bradi5g03300 Fg don+ vs Tween 7.46 0.00 12.01 0.16 UDP-glycosyltransferase
  Fg don- vs Tween 0.88 1.00 2.92 0.47  
  Fg don+ vs Fg don- 6.59 0.00 9.08 0.16  
Bradi5g03370 Fg don+ vs Tween 6.61 0.00 9.51 0.19 UDP-glycosyltransferase
  Fg don- vs Tween 2.09 0.00 3.29 0.44  
  Fg don+ vs Fg don- 4.52 0.00 6.22 0.19  
Bradi5g03380 Fg don+ vs Tween 6.72 0.00 12.46 0.22 UDP-glycosyltransferase
  Fg don- vs Tween 1.52 0.01 4.36 0.73  
  Fg don+ vs Fg don- 5.20 0.00 8.10 0.22  
Bradi5g03390 Fg don+ vs Tween 4.34 0.00 6.23 0.08 UDP-glycosyltransferase
  Fg don- vs Tween 1.86 0.00 2.15 0.44  
  Fg don+ vs Fg don- 2.49 0.00 4.08 0.08  
Bradi5g03400 Fg don+ vs Tween 1.22 1.00 2.51 0.13 UDP-glycosyltransferase
  Fg don- vs Tween 1.37 0.14 2.01 0.10  
  Fg don+ vs Fg don- -0.16 1.00 0.50 0.13  
Bradi2g04720 Fg don+ vs Tween 5.41 0.00 8.76 0.20 UDP-glycosyltransferase
  Fg don- vs Tween 3.22 0.00 5.47 0.24  
  Fg don+ vs Fg don- 2.19 0.00 3.29 0.20  
Bradi2g04760 Fg don+ vs Tween 4.71 0.00 7.25 0.19 UDP-glycosyltransferase
  Fg don- vs Tween 1.04 1.00 2.57 0.26  
  Fg don+ vs Fg don- 3.67 0.00 4.68 0.19