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Figure 3 | BMC Genomics

Figure 3

From: Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing

Figure 3

Dual indexing in a 4-primer approach. The 2 outer and 2 inner primers are combined in one PCR reaction to yield a MiSeq compatible product with dual indexing. Each sample is first mixed with a unique combination of outer primer indexes. The 48 samples are then combined with up to 48 target-specific primer sets in 2304 separate reaction chambers on the Fluidigm chip for PCR.

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