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Table 1 Resolution in comparison with Sanger based sequencing

From: Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing

Locus

Sanger

NGS

A

90.4%

99.8%

B

95.0%

97.6%

C

92.3%

95.7%

DPB1

99.8%

99.9%

DQB1

95.7%

100.0%

DRB1

96.6%

100.0%

Average

95.0%

98.8%

  1. Rate of high resolution typing results. Resolution of 37,975 samples typed by the proposed NGS approach in September 2013 was compared with 145,932 samples analyzed by Sanger sequencing in 2012. In accordance with the standards of the European Federation for Immunogenetics (EFI), we refer to allele typing results with no ambiguities in the antigen recognition site as “high resolution”.
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BMC Genomics

ISSN: 1471-2164

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