Skip to main content
Figure 2 | BMC Genomics

Figure 2

From: Analysis of stranded information using an automated procedure for strand specific RNA sequencing

Figure 2

Quality control metrics for human cell line libraries. a) rRNA content in libraries treated with RiboZero is 2.24% on average while rRNA content in libraries treated with RiboMinus is 65.7% on average. Error bars denote standard error. b) The strand specificty of strand specific libraries is 96.6% on average; the libraries treated with RiboMinus have slightly higher strand specificity than the libraries treated with RiboZero. The unstranded libraries have strand specificity of 50.0%. c) The duplication rate varies between the libraries. The higher duplication rate of the libraries treated with RiboZero compared to the libraries treated with RiboMinus can partly be explained by the much higher sequencing depth of those libraries. The hollow triangles represent the duplication rate of the downsampled data (see text for details). d) All libraries show even gene coverage. The percentages in parenthesis is the percentage of reads that map closer to the 3’ end than to the 5’ end. [RM = RiboMinus, RZ = RiboZero, SS = strand specific, NS = non-stranded].

Back to article page