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Figure 5 | BMC Genomics

Figure 5

From: Assessing the hodgepodge of non-mapped reads in bacterial transcriptomes: real or artifactual RNA chimeras?

Figure 5

Comparison between different methodologies to prepare RNA-seq libraries. A) Schematic representation of the Directional RNA-seq and TruSeq Stranded RNA-seq protocols. B) Histogram representing the percentage of reads that do not map against M. pneumoniae genome in the different protocols used to prepare RNA-seq libraries. C) The inverted pyramids indicate the different number of chimeric RNAs obtained in the consecutive steps of sample validation for both protocols of library preparation.

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