Experiment validation of sncRNAs in Cassava cultivar SC124. (A) Expression of miRNAs confirmed by qRT-PCR validation, where “selected” is the percent of miRNAs selected for qRT-PCR assays from all expressed miRNAs based on sequencing data, and “Detected” is the percent of the selected miRNAs that can be detected by qRT-PCR in at least one of the four conditions. (B) Examples of validated known and novel miRNAs expressed in three chilling treatments and the normal condition. (C) Amplification curve, dissolution curve, and endpoint gel image of amplicon products of miRNA novel-55. Solid arrows indicate the target band in gel. (D) The hairpin structure of novel-55 with annotated miRNA-5p highlighted in blue. The number in parenthesis represents the number of reads in the sequencing data.