Figure 2

miR390-triggered siRNAs from TAS3c in Cassava. (A) Distribution of small-RNA reads. Plotted are the 5′ ends of the reads at each position along the sense (in blue) and antisense (in red) strands within the TAS3c region. The two arrows marked with miR390 indicate the cleavage sites of miR390 on TAS3c.The distribution of the lengths of these small RNAs is shown in the right-top figure. The radial graph in the right-bottom shows the percent of small RNAs with 5′ ends aligned to one of the 21-phasing registers. The registers to which 5′ and 3′ miR390 cleavage sites are aligned are indicated by miR390 5′ and miR390 3′. (B) The two target sites (top) and base pairing of miR390 (bottom) on TAS3c in Cassava. Sense (in blue) and antisense reads (in red) alignments are plotted near the two target sites. The arrows indicate the putative cleavage sites. Phased siRNA reads duplex with ~2 nt 3′ overhang can be observed on the sense (blue) and antisense (red) strands. The yellow colored are the miR390 targets. (C) Conservation of TAS3c between Cassava and Castor bean. The regions marked blue are conserved with identical bases in the two species.