Figure 3

Cleavage sites of ta-siRNAs detected by 5′-RACE in chilling-treated Cassava plants. Shown here are the cleavage sites of (A) tasiARF3 on ARF3 (002399 m), (B) tasiARF1 on ARF3 (002399 m), (C) tasiARF2 on NAC2 (027253 m), and (D) tasiARF1 on ARF4 (001979 m). In each panel, the name of a target, its annotation and the ta-siRNA-binding region are shown on the top and the reversed ta-siRNA sequence on the bottom with conditions shown to the left (CA, CCA and/or CS). An arrow indicates a cleavage on the target gene. The first number atop of an arrow refers to the cleavage site within the ta-siRNA-binding region from the 5′ end of the small RNA, and the following number is the number of clones (out of 10) detected by cloning of amplified the specific PCR product. A number with prefix “+” or “-” refers to, respectively, the cleavage site located upstream or downstream of the base-pairing region on the target gene. The number on a solid line indicates the distance between the cleavage site and the boundary of ta-siRNA-binding region.