Figure 2

Immunoreactivity of recombinant HOXC11 protein. A) ELISA: Immunoreactivity of refolded HOXC11 was analyzed by primary antibody (rabbit anti-HOXC11) and secondary antibody (HRP labelled goat anti-rabbit IgG). Optical density was taken at 492 nm. N indicates neat protein and N/2 to N/128 represent the different dilutions of HOXC11 protein. B) Western blot for purified protein using specific antibodies against HOXC11 protein. Note the 33 kDa band in the induced sample confirming the purified protein.