Figure 5
From: Prediction of miRNA-mRNA associations in Alzheimer’s disease mice using network topology
Validation of ARRDC3 function in primary cultured cortical neurons from Tg6799 transgenic mice. (A) Overexpression of ARRDC3 gene in primary cultured cortical neurons from Tg6799 mice. RT-PCR results show that the mRNA levels of PPP1R3C and Sfpq were increased by ARRDC3 overexpression. (B) Knock-down of ARRDC3 gene in primary cultured cortical neurons from Tg6799 mice. The mRNA levels of PPP1R3C and Sfpq were decreased by the treatment of ARRDC3 siRNA. (C) Real-time PCR data show that the expression levels of miR139-5p and miR3470a had decreasing tendency in the primary cortical neurons of Tg6799 mice. (D) Immunocytochemical staining show no significant enhancement in the number and length of neurites in ARRDC3 overexpressed primary cortical neurons from Tg6799 mice (§: p < 0.05 between LM and MT mice).