Figure 1

Gene expression analysis of A. pisum sugar transporters. (A) Sugar transporter gene expression levels were obtained using real-time quantitative PCR (QPCR) in whole adult females (WI), embryo (E), head (H) and gut (G) tissues. Gene expression was normalized to GAPDH and compiled into a heat map, showing high expression (z-score = 10, yellow) to low expression (z-score = −10, blue). Transporter gene expression profiles are ranked from high whole insect (WI) expression (ACYPI# 4204) to low WI expression (ACYPI# 9298). (B and C) Sugar transporter expression level in whole insects (B) and gut tissue (C) relative to GAPDH (GAPDH = 1, grey dashed line). Only transporters >10% GAPDH expression are shown (n = 3, with error bars representing ± SEM). (D) Transporters enriched > 2-fold in gut tissue relative to whole insect (WI) are indicated with confidence limits [15] in parentheses (n = 3). Transporter identification numbers (ApST#) are as previously described [13]. All transporter sequences are available from NCBI by appending ACYPI00 to each of the ACYPI#.