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Table 1 Descriptive statistics of the reference genes Cq values

From: Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset

Gene

N

Mean

SD

Min Cq

Max Cq

KS-test p

ACTB

212

15.87

1.21

13.52

19.02

0.197

B2M

212

19.50

1.70

16.51

24.44

0.009

GAPDH

212

20.18

1.65

16.81

24.72

0.477

RPL17

212

18.44

1.46

15.67

22.70

0.130

RPS4

212

21.21

1.65

17.82

25.43

0.739

UBQ

212

18.34

1.12

15.68

21.09

0.108

  1. Number of samples (N), mean, Standard deviation (SD), Minimum Cq value (Min Cq), Maximum Cq value (Max Cq) and p value of the Kolmogorov-Smirnov test (KS-test p) are shown for each candidate reference gene.
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BMC Genomics

ISSN: 1471-2164

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