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Table 10 Efficiency-corrected delta Cqs by sex group with each efficiency + reference gene combination

From: Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset

 

Female Cq Mean

Female Cq SD

Male Cq Mean

Male Cq SD

CYP19a UBQ + RPS4 LinRegPCR

-2.82

2.39

4.37

3.17

CYP19a UBQ + RPS4 PCR-Miner

-3.03

2.57

4.7

3.41

CYP19a B2M LinRegPCR

-3.05

2.67

4.74

3.99

CYP19a B2M PCR-Miner

-3.28

2.87

5.1

4.29

AMH UBQ + RPS4 LinRegPCR

0.85

3.24

-1.32

3.54

AMH UBQ + RPS4 PCR-Miner

0.92

3.49

-1.42

3.8

AMH B2M LinRegPCR

0.59

3.26

-0.91

3.69

AMH B2M PCR-Miner

0.63

3.51

-0.97

3.95

SOX19 UBQ + RPS4 LinRegPCR

-2.31

2.54

3.58

1.25

SOX19 UBQ + RPS4 PCR-Miner

-2.47

2.71

3.84

1.34

SOX19 B2M LinRegPCR

-2.54

3.73

3.95

1.59

SOX19 B2M PCR-Miner

-2.73

4

4.23

1.72

SOX9 UBQ + RPS4 LinRegPCR

0.67

1.46

-1.04

0.95

SOX9 UBQ + RPS4 PCR-Miner

0.72

1.57

-1.12

1.01

SOX9 B2M LinRegPCR

0.44

1.72

-0.68

2.07

SOX9 B2M PCR-Miner

0.47

1.85

-0.73

2.23

SOX17 UBQ + RPS4 LinRegPCR

-0.98

1.1

1.53

1.62

SOX17 UBQ + RPS4 PCR-Miner

-1.05

1.17

1.63

1.73

SOX17 B2M LinRegPCR

-1.22

2.35

1.89

2.39

SOX17 B2M PCR-Miner

-1.31

2.52

2.03

2.57

VASA UBQ + RPS4 LinRegPCR

-0.17

3.2

0.27

2.87

VASA UBQ + RPS4 PCR-Miner

-0.19

3.44

0.29

3.08

VASA B2M LinRegPCR

-0.41

3.09

0.63

2.98

VASA B2M PCR-Miner

-0.44

3.32

0.68

3.2

  1. Mean efficiency-corrected delta Cqs and standard deviation (SD) values for males and females in the four datasets produced after efficiency correction with LinRegPCR or PCR-Miner and later normalization with UBQ + RPS4 or B2M.