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Table 10 Efficiency-corrected delta Cqs by sex group with each efficiency + reference gene combination

From: Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset

  Female Cq Mean Female Cq SD Male Cq Mean Male Cq SD
CYP19a UBQ + RPS4 LinRegPCR -2.82 2.39 4.37 3.17
CYP19a UBQ + RPS4 PCR-Miner -3.03 2.57 4.7 3.41
CYP19a B2M LinRegPCR -3.05 2.67 4.74 3.99
CYP19a B2M PCR-Miner -3.28 2.87 5.1 4.29
AMH UBQ + RPS4 LinRegPCR 0.85 3.24 -1.32 3.54
AMH UBQ + RPS4 PCR-Miner 0.92 3.49 -1.42 3.8
AMH B2M LinRegPCR 0.59 3.26 -0.91 3.69
AMH B2M PCR-Miner 0.63 3.51 -0.97 3.95
SOX19 UBQ + RPS4 LinRegPCR -2.31 2.54 3.58 1.25
SOX19 UBQ + RPS4 PCR-Miner -2.47 2.71 3.84 1.34
SOX19 B2M LinRegPCR -2.54 3.73 3.95 1.59
SOX19 B2M PCR-Miner -2.73 4 4.23 1.72
SOX9 UBQ + RPS4 LinRegPCR 0.67 1.46 -1.04 0.95
SOX9 UBQ + RPS4 PCR-Miner 0.72 1.57 -1.12 1.01
SOX9 B2M LinRegPCR 0.44 1.72 -0.68 2.07
SOX9 B2M PCR-Miner 0.47 1.85 -0.73 2.23
SOX17 UBQ + RPS4 LinRegPCR -0.98 1.1 1.53 1.62
SOX17 UBQ + RPS4 PCR-Miner -1.05 1.17 1.63 1.73
SOX17 B2M LinRegPCR -1.22 2.35 1.89 2.39
SOX17 B2M PCR-Miner -1.31 2.52 2.03 2.57
VASA UBQ + RPS4 LinRegPCR -0.17 3.2 0.27 2.87
VASA UBQ + RPS4 PCR-Miner -0.19 3.44 0.29 3.08
VASA B2M LinRegPCR -0.41 3.09 0.63 2.98
VASA B2M PCR-Miner -0.44 3.32 0.68 3.2
  1. Mean efficiency-corrected delta Cqs and standard deviation (SD) values for males and females in the four datasets produced after efficiency correction with LinRegPCR or PCR-Miner and later normalization with UBQ + RPS4 or B2M.