Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset

Table 11 Primer table

Gene name	Accession ID	Primer F (5’ - > 3’)	Primer R (5’ - > 3’)	Product Length (bp)
RPS4	FE943956	CAACATCTTCGTCATCGGCAAGG	ATTGAACCAGCCTCAGTGTTTAGC	143
RPL17	DQ848879	ACCAGTGCGTCCCCTTCA	CTCATCTTCGGAGCCTTGTTC	214
GAPDH	FE950888	CGCCCATAGCCCAGTCATAGC	TGGCAGAGGGAGGTGGAGAG	167
ACTB	EU686692	GTAGGTGATGAAGCCCAGAGCA	CTGGGTCATCTTCTCCCTGT	204
UBQ	FE946708	GCGTGGTGGCATCATTGAGC	CTTCTTCTTGCGGCAGTTGACAG	124
B2M	DQ848854	CTCTGGCTGTTTTCGTCTGCT	TCCTTTCCGTTCTCTCCCG	86
CYP19a	JQ403643	CAGCGAGGAAGCTGGCAAACA	ACACGCAGACTCGGCTTTTTACATC	148
AMH	JQ403642	CCAGGGCGGACCCCGATAAC	TGGCTGTGTTTGGACCCACGAG	99
SOX9	JQ300535	ATCAGTACCCACACCTGCATAAC	TCAGCCTCCTCCACGAACG	103
SOX19	JQ403639	ACCGAGCGGTTTGTGCCTTG	TCCTCTGGATGCAGTGCTGATTGT	122
SOX17	JQ403638	TGTTCGGGAAGCAGGTGAAAGGT	CTTGTTGCCATTTTAGGGGACAGT	92
VASA	JX235364	CTTAGCTGTGGGCGTGGTGGG	ACGTTCTCCTGGCACATCAACG	190

Gene name, accession number, primer sequences and amplicon size of the reference genes (RPS4, RPL17, GAPDH, ACTB, UBQ, B2M) and the target genes (CYP19a, AMH, SOX9, SOX19, SOX17, VASA) are shown.

ISSN: 1471-2164