Skip to main content

Table 3 Stability rankings obtained with the different reference gene determination methods

From: Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset

Rank Comparative Delta-Ct BestKeeper (SD) BestKeeper (CV%) NormFinder GeNorm
1 UBQ (1.267) UBQ (1.12) UBQ (4.96) RPS4 (0.613) UBQ/RPS4 (0.952)
2 RPS4 (1.278) ACTB (1.21) ACTB (6.16) UBQ (0.713)  
3 RPL17 (1.323) RPL17 (1.46) RPL17 (6.34) RPL17 (0.721) RPL17 (1.154)
4 ACTB (1.381) RPS4 (1.65) RPS4 (6.43) ACTB (0.785) ACTB (1.202)
5 GAPDH (1.431) GAPDH (1.66) GAPDH (6.66) GAPDH (0.85) GAPDH (1.290)
6 B2M (1.52) B2M (1.70) B2M (7.43) B2M (0.851) B2M (1.367)
  1. Stability values obtained by each method are shown in parenthesis for each candidate reference gene. Both Standard deviation (SD) and Coefficient of variation (CV) rankings are shown for BestKeeper. The genes are ranked from most stable (1) to least stable (6).