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Table 5 Standard deviation for target genes when normalized by different gene combinations

From: Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset

 

Average intragroup SD

Average intergroup SD

Average male group SD

Average female group SD

UBQ

1.53

2.83

2.48

2.57

RPS4

1.49

2.69

2.50

2.63

UBQ + RSP4

1.47

2.71

2.47

2.57

ACTB + RPS4

1.53

2.79

2.63

2.8

UBQ + RPS4 + RPL17

1.50

2.73

2.53

2.66

UBQ + RPS4 + ACTB

1.48

2.78

2.54

2.68

UBQ + RPS4 + RPL17 + ACTB

1.50

2.74

2.61

2.78

  1. Intragroup and intergroup normalized Cq standard deviations (SD) averaging the results for the six target genes are shown for Fish age + Rearing temperature groups when normalized by different candidate reference gene combinations. Standard deviations (SD) for males and females when normalized by the same combinations are also shown.
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