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Table 5 Standard deviation for target genes when normalized by different gene combinations

From: Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset

  Average intragroup SD Average intergroup SD Average male group SD Average female group SD
UBQ 1.53 2.83 2.48 2.57
RPS4 1.49 2.69 2.50 2.63
UBQ + RSP4 1.47 2.71 2.47 2.57
ACTB + RPS4 1.53 2.79 2.63 2.8
UBQ + RPS4 + RPL17 1.50 2.73 2.53 2.66
UBQ + RPS4 + ACTB 1.48 2.78 2.54 2.68
UBQ + RPS4 + RPL17 + ACTB 1.50 2.74 2.61 2.78
  1. Intragroup and intergroup normalized Cq standard deviations (SD) averaging the results for the six target genes are shown for Fish age + Rearing temperature groups when normalized by different candidate reference gene combinations. Standard deviations (SD) for males and females when normalized by the same combinations are also shown.