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Figure 1 | BMC Genomics

Figure 1

From: Comparison of gene expression microarray data with count-based RNA measurements informs microarray interpretation

Figure 1

Median and variance properties of expressed versus unexpressed genes and interplatform correlation. A) Genes are plotted by cell-type-specific RMA + ComBat- (CD4 and CD14) or RMA-preprocessed (CD16) microarray expression value median and variance. Red indicates genes called unexpressed by nCounter measurement. B) nCounter datasets were normalized to two cell-type-specific control genes each, log-transformed and compared to RMA + ComBat- (CD4 and CD14) or RMA-preprocessed (CD16) microarray data without control-gene normalization. Plots show Pearson correlation of each expressed gene versus the median or variance of its microarray expression values or control-gene-normalized, log-transformed nCounter measurements: blue = CD4, green = CD14, red = CD16 datasets. C) RMA + ComBat- (CD4 and CD14) or RMA-preprocessed (CD16) microarray data plotted versus nCounter measurements normalized to two cell-type-specific control genes.

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BMC Genomics

ISSN: 1471-2164

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