Autolytic and virulence capacities of knockouts. Vancomycin (A), penicillin (B) and DOC (C) treatments. DOC assays were performed with (for 5 min) and without (for 30 min) the addition of exogenous LytA. Means ± SD for experiments performed in triplicate for (A) and (B), and in duplicate for (C), are shown. Colours for protein classes are as described in the legend to Figure 5. (D) Virulence capacity of knockouts. Grey bars represent the average CI with respect to the D39 strain in an in vivo sepsis model. Diamonds represent the CI value of a single mouse. Values 10-fold higher or lower than the average (white diamonds) were not considered in calculations of the average CI (up to a maximum of 2 mice per knockout).